|
|
|
| |
PHYLOGENETIC ANALYSIS OF BIOLUMINESCENT ORGANISM |
| |
-
Maruthamuthu Rajadurai*, Gopalakrishnan
Vidhya, Mohandass Ramya and Bhaskar Anusha
-
PG Department of Biotechnology and Bioinformatics,
Bishop Heber College,
Tiruchirappalli 620 017.
-
*Author correspondence: Phone: 0431-2770136
Extn: 404/405
E-Mail -mr.rajadurai@gmail.com
|
| |
ABSTRACT |
| |
Luciferin is a chemical substance, which
emits light in the presence of luciferase enzyme. The bioluminescent
cells produce some form of luminescence within the electromagnetic
spectrum, which may or may not be visible to the naked eye.
This property is unique in wavelength, duration, timing, and
regulatory of flashes. This uniqueness of luciferase found in
various bioluminescent organisms, can be analyzed to study their
evolutionary existence by using Prot Param, ClustalX, NJ plot
and FM method. |
| |
SYNERGISTIC EFFECT OF ETHANOLIC LEAF EXTRACTS
OF SELECTED PLANT SPECIES AGAINST BACTERIA |
| |
-
Karthikeyan .V*, M. Ravi kumar, N. Karmegam,
M. Saravanan and C.R. Sai Rajasekar
-
Karpaga Vinayaga College of Engineering
& Technology, G.S.T.Road, Chinna kolambakkam, Kanchipuram-district,
Tamil nadu, India.
-
*Author correspondence: Mobile:9865224289,
E-mail karthimicro2006@yahoo.co.in
|
| |
ABSTRACT |
| |
Ethanolic leaf extracts of six different
groups of medicinal and aromatic plants (Aegle marmelos, Eucalyptus
globulus, Centella asiatica, Lawsonia inermis, Catharanthus
roseus and Chrysanthemum odoratum) are prepared and the sensitivity
of selected test microorganisms to the ethanolic leaf extracts
is determined. Different combinations of ethanolic leaf extracts
of plants show effective antimicrobial activity against Gram
positive and Gram negative bacteria. The synergistic effect
is more when the ethanolic leaf extracts are used in combination
than when it is used alone. Combinations of Catharanthus roseus,
Chrysanthemum odoratum and Aegle marmelos show enhanced antimicrobial
activity against the selected microbes |
| |
EVALUATION OF WOUND HEALING ACTIVITY OF POLYGONUM
BARBATUM LINN IN WISTAR ALBINO RATS |
| |
-
Nithya V*,Sr. Lecturer, Department of
Biochemistry, Srimad Andavan Arts and Science College, Tiruchirappalli-620
005.
-
Balasubramanian.K., Head, Department
of Chemistry, National College, Tiruchirappalli-620 001.
-
*Author correspondence: Mobile-9442200888,
E-mail: nithya.gopinath@rediffmail.com
|
| |
ABSTRACT |
| |
A study was conducted to evaluate the wound
healing activity of Polygonum Barbatum Linn on albino rats of
Wistar strain using two different models viz, excision and dead
space wound. There was a significant increase in wound closure
rate, tensile strength, dry granuloma weight, wet granuloma
weight and decrease in epithelization period in Polygonum Barbatum
Linn treated group when compared to control and commercial drug-treated
groups. From the results, it may be concluded that, the ethanolic
extract of Polygonum Barbatum Linn had greater wound - healing
activity than the nitrofurazone ointment. |
| |
Efficacy of Ferrous Iron on Cellulolytic Activity
of Pestalotia psidii |
| |
-
R. SENTHIL KUMAR*,P.G. and Research Department
Microbiology, J.J. College of Arts and Science, Pudukkottai-622422,
Tamil Nadu, India.
-
A. PANNEERSELVAM, P.G. and Research Department
of Botany and Microbiology, A.V.V.M. Sri Pushpam College
(Autonomous) Poondi-613 503,Thanjavur, Tamil Nadu, India.
-
N. THAJUDDIN,Department of Microbiology,
Bharathidasan, University, Tiruchirappalli-620 024, Tamil
Nadu, India.
-
R. SARAVANA MUTHU,Department of Botany,
A.V.C. College, Mayiladuthurai-609 305, Tamil Nadu, India.
-
*Author correspondence: Mobile: 9865412467
E-mail: drmicrsk@yahoomail.com
|
| |
ABSTRACT |
| |
Cellulolysis rate of the pathogenic fungus,
Pestalotia psidii, was determined by estimating the loss in
dry weight of filter paper. It varied in relation to changing
ferrous iron content of the nutrient solution. There was positive
correlation between the growth rate of the fungus and the mean
loss in dry weight of the filter paper discs. |
| |
In-vitro propagation of Pterospermum xylocarpum
(Sterculiaceae) |
| |
-
K.R.S. Sambasiva Rao* ,Department of
Biotechnology,JC College of Engineering, Guntur, A.P., India
-
A. Krishna Satya,Centre for Biotechnology,
Acharya Nagarjuna University
Nagarjunanagar – 522 510, Guntur, A.P., India.
-
P. Krishna Kanth,Department of Biotechnology,RV
R & JC College of Engineering, Guntur, A.P., India
-
*Author Correspondence: E-mail- krssrao@gmail.com
|
| |
ABSTRACT |
| |
The present study is focused
on in-vitro propagation of a threatened plant of Andhra Pradesh
–
Pterospermum xylocarpum (Sterculiacea). Lack of organized cultivation
of these plants demand immediate conservative measures. The
objectives of the present study are to standardize in-vitro
germination techniques, developing a proper in-vitro regeneration
protocol using suitable seedling explants, and to obtain organogenesis.
The process of micro propagation was carried out by using in-vitro
grown seedling explants. Germination studies were carried out
to indentify this best medium for developing a healthy seedling.
Explant evolution was done upon the shoot regenerative capacity
of the inoculated seedling explants. Selected explants were
subjected to media evaluation. Morphogenic response of the explants
were checked on MS media supplemented with cytokinin –
like N6 – Benzylaminopurine (BAP), Kinetin (KN), Thidiazuron
(TDZ) and Auxin – like Indole acetic acid (IAA), Naphthalene
acetic acid (NAA) and Indole butyric acid (IBA) were supplemented
either individually or in combination. The regenerated shoots
were separated and placed on the rooting medium augmented with
different Auxins like IAA, IBA & NAA (0.1-2 mgL-1). IAA
(2 mgL-1) to induce roots from the cut end of the micro shoots.
Rooted plantlets were acclimatized to field conditions by placing
in pots containing sterilized sand. Cotyledonary nodes of Pterospermum
xylocarpum are the best explants for in-vitro propagation. MS
medium with 2-3% sucrose was found to be most suitable. Shoot
proliferation was maximum in MS medium reinforced with BAP 2mgL-1
+ KN 1mgL-1 + 3% sucrose solution. Adventitious rooting was
accelerated in micro shoots with IAA at 2mgL-1. Shoot buds are
produced from the callus of shoot tips in MS medium reinforced
with BAP 4mgL-1 and IAA 1mgL-1.
|
| |
PREVALENCE OF MICROBIAL INHABITANTS FROM COWSHED
AND ITS CONTROL |
| |
-
Bhakyaraj R*, , Arun Sasi, N. Yogananth,
A. Chanthuru,
P.G. and Research Department of Microbiology, J.J. College
of Arts and Science,
Pudukkottai- 622 404, Tamil Nadu, India.
- J. Vetriselvi,Department of Microbiology, Sengamalathayar
College of Arts and Science (Women) Mannarkudi, Tamil Nadu,
India.
- S. Palanivel,Department of Botany, Govt. College of Arts
and Science, Karur, Tamil Nadu, India.
- * Author correspondence: E-mail: rbrajbhakya@gmail.com
|
| |
ABSTRACT |
| |
Cow dung contains vast quantities of pathogenic
bacteria and fungi. Totally four bacterial strains viz; Streptococcus
sp, Bacillus sp, Clostridium sp, Staphylococcus sp and five
fungal strains viz; Fusarium semitectum, Aspergillus flavus,
Aspergillus versicolor, Aspergillus ustus, Aspergillus sydowi
were isolated from cow dung with soil and two dominant types
of bacteria i.e., Streptococcus sp, Bacillus sp and two fungi
i.e., Fusarium semitectum, Aspergillus flavus were chosen for
treatment with Na2Co3¬. The experimental group showed a
higher inhibition spectra in both bacterial and fungal colonies
compared with those in the to control groups. |
| |
Studies on Production of antibiotic from Tsunami soil isolates
of Streptomyces sp at Velanganni, Nagapattinam District |
| |
- Chanthuru A*, N.Yogananth, R.Bhakyaraj, Arun Sasi
P.G. and Research Department of Microbiology, J.J. college
of Arts and Science, Pudukkottai- 622 422, Tamil Nadu, India.
- S.Palanivel,Lecturer, Department of Botany, H.H. Rajah’s
college for Men, Pudukkottai, Tamil Nadu, India.
- *Author for correspondence: E-mail: bioyogaa@gmail.com E-mail:
ssspvrm@gmail.com
|
| |
ABSTRACT |
| |
Tsunami is usually generated by Sea-floor
disturbances associated with volcanic eruptions or land slides.
Soil samples were collected from tsunami affected areas and
their physico-chemical characters analysed to assess the distribution
of bacteria and fungi. It was observed that Tsunami soil has
toxic chemicals such as Fe, Pb, Hg etc. A total of 5 genera
of bacteria viz; Escherichia coli, Staphylococcus aureus, Pseudomonas
aeruginosa, Bacillus cereus and Bacillus subtilis and one fungi,
Candida albicans were isolated. In addition to these, Actinomycetes
members were also enumerated. The chloroform extracts of Streptomyces
culture filtrates (50 ppm) were screened for antimicrobial activity.
Two streptomyces isolates were used. It was observed that Candida
albicans and Pseudomonas aeruginosa were highly sensitive (0.0
to 58.0 mm and 25.00 to 60.81 mm respectively), followed by
Bacillus cereus (28 to 42 mm) and Staphylococcus aureus (27.0
to 39.62 mm). Inhibitory activity of these isolates appeared
to be high for Escherichia coli (10.00 to 30.62 mm) when compared
to Bacillus subtilis (11 to 28.62 mm). |
| |
| |
DOES CADMIUM TOXICITY INDUCE OXIDATIVE STRESS IN CHICKPEA (CICER ARIETINUM. L)? |
| |
-
D Amirtham* and Dr V N Satakopan
Department of Biochemistry, PSG College of Arts & Science
Coimbatore, Tamil Nadu, 641014, India
- *Author for correspondence: E-mail:amirtham_d@yahoo.com
|
| |
ABSTRACT |
| |
This investigation was carried out to verify
whether Cadmium toxicity could cause oxidative stress in Chickpea.
The data indicated decreased germination percentage, root length,
shoot length and protein level. Significant accumulation of
Proline, an indicator of water stress occurred in the presence
of Cadmium along with a gradual increase in the activity of
antioxidant enzyme SOD. Increased lipid peroxidation (TBARS)
was suggestive of disturbed oxidative metabolism. Cadmium caused
a transient depletion of Peroxidase and vitamin C in root, shoot
and cotyledon. The data also confirmed the inhibitory effect
of Cadmium on Glutathione reductase and Glucose 6-phosphate
dehydrogenase activities especially in the root system of chickpea.
But there is a modified pattern of Gutathione reductase and
Glucose 6-phosphate dehydrogenase activity was found in the
shoot and cotyledon system suggesting the possibility for the
presence of isoenzymes. There was no significant change in the
shoot Catalase activity but the root and cotyledon Catalase
activities were found to be significantly decreased in presence
of Cadmium. Results obtained suggested an induction of oxidative
stress in germinating chickpea under Cadmium phytotoxicity. |
| |
Production of Alpha-amylase enzyme in submerged fermentation
by using Bacillus sp |
| |
-
Arun Sasi*, Bhakyaraj. R, Yogananth.
N, Chanthuru. A,
P.G. & Research Department of Microbiology
J.J. College of Arts and Science, Pudukkottai – 622
422.
-
Ravikumar. M ,Department of Plant Biology
and Plant Biotechnology Govt. Arts College for Men, Nandanam,
Chennai.
-
*Author correspondence: E-mail- vavamicro@gmail.com
|
| |
ABSTRACT |
| |
Amylases (endo-1,4?-D glucan glycohydrolases
EC 3.2.1.1) are enzymes that hydrolyze starch. They can be derived
from several sources such as plants, animals and microorganisms.
They are used in food, baking, brewing, detergent, textile,
paper and distilling industries. ?-amylase enzyme can be produced
in submerged fermentation by using Bacillus substilis and Bacillus
lencheniformis. The Minimal medium was supplemented with certain
carbon sources like fructose, glucose, lactose, mannitol, mannose
and sucrose, certain other nitrogen compounds like peptone,
urea, (NH4)2 SO4, NaNO3 and yeast extracts and certain heavy
metal compounds like Ca 2+, Mg 2+, Zn 2+, Mn 2+, Fe 2+ and Co
2+. In this study it was found that B. substilis produced more
amounts of amylase (278 ?l –1) than B. lencheniformis
in both normal and supplemented media in 37°C at pH 7. |
| |
Deterioration of fabric paints by two species of Aspergillus, Tham and Raper. |
| |
|
| |
ABSTRACT |
| |
Aspergillus flavus and Aspergillus .fumigatus
were isolated from spoilt black and yellow fabric paints respectively.
The capacity of these two fungi to utilize fabric paints for
their growth was studied by inoculating them on fresh paints
and on Czapex-Dox medium with and without sucrose but containing
50, 250 and 500 ppm concentrations of black and yellow fabric
paints. Both fungi could grow to some extent directly on fresh
black and yellow fabric paints. In Czapek-Dox medium with sucrose
and 50ppm concentration of either black or yellow fabric paints
they grew well and sporulated profusely. In Czapek-Dox medium
without sucrose but with different concentrations of paints
as carbon source the fungi showed slightly more growth than
the control indicating that they utilize the chemicals present
in paints. Zinc oxide, a common fungistatic substance commonly
used in paints was found to be ineffective in controlling A.flavus
and A.fumigatus at the concentration routinely used in paints. |
 |
| |
|
