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PHYLOGENETIC ANALYSIS OF BIOLUMINESCENT ORGANISM

 
  • Maruthamuthu Rajadurai*, Gopalakrishnan Vidhya, Mohandass Ramya and Bhaskar Anusha
  • PG Department of Biotechnology and Bioinformatics, Bishop Heber College,
    Tiruchirappalli 620 017.
  • *Author correspondence: Phone: 0431-2770136 Extn: 404/405
    E-Mail -mr.rajadurai@gmail.com
 

ABSTRACT

 
Luciferin is a chemical substance, which emits light in the presence of luciferase enzyme. The bioluminescent cells produce some form of luminescence within the electromagnetic spectrum, which may or may not be visible to the naked eye. This property is unique in wavelength, duration, timing, and regulatory of flashes. This uniqueness of luciferase found in various bioluminescent organisms, can be analyzed to study their evolutionary existence by using Prot Param, ClustalX, NJ plot and FM method.
 

SYNERGISTIC EFFECT OF ETHANOLIC LEAF EXTRACTS OF SELECTED PLANT SPECIES AGAINST BACTERIA

 
  • Karthikeyan .V*, M. Ravi kumar, N. Karmegam, M. Saravanan and C.R. Sai Rajasekar
  • Karpaga Vinayaga College of Engineering & Technology, G.S.T.Road, Chinna kolambakkam, Kanchipuram-district, Tamil nadu, India.
  • *Author correspondence: Mobile:9865224289, E-mail karthimicro2006@yahoo.co.in
 

ABSTRACT

 
Ethanolic leaf extracts of six different groups of medicinal and aromatic plants (Aegle marmelos, Eucalyptus globulus, Centella asiatica, Lawsonia inermis, Catharanthus roseus and Chrysanthemum odoratum) are prepared and the sensitivity of selected test microorganisms to the ethanolic leaf extracts is determined. Different combinations of ethanolic leaf extracts of plants show effective antimicrobial activity against Gram positive and Gram negative bacteria. The synergistic effect is more when the ethanolic leaf extracts are used in combination than when it is used alone. Combinations of Catharanthus roseus, Chrysanthemum odoratum and Aegle marmelos show enhanced antimicrobial activity against the selected microbes
 

EVALUATION OF WOUND HEALING ACTIVITY OF POLYGONUM BARBATUM LINN IN WISTAR ALBINO RATS

 
  • Nithya V*,Sr. Lecturer, Department of Biochemistry, Srimad Andavan Arts and Science College, Tiruchirappalli-620 005.
  • Balasubramanian.K., Head, Department of Chemistry, National College, Tiruchirappalli-620 001.
  • *Author correspondence: Mobile-9442200888, E-mail: nithya.gopinath@rediffmail.com
 

ABSTRACT

 
A study was conducted to evaluate the wound healing activity of Polygonum Barbatum Linn on albino rats of Wistar strain using two different models viz, excision and dead space wound. There was a significant increase in wound closure rate, tensile strength, dry granuloma weight, wet granuloma weight and decrease in epithelization period in Polygonum Barbatum Linn treated group when compared to control and commercial drug-treated groups. From the results, it may be concluded that, the ethanolic extract of Polygonum Barbatum Linn had greater wound - healing activity than the nitrofurazone ointment.
 

Efficacy of Ferrous Iron on Cellulolytic Activity of Pestalotia psidii

 
  • R. SENTHIL KUMAR*,P.G. and Research Department Microbiology, J.J. College of Arts and Science, Pudukkottai-622422, Tamil Nadu, India.
  • A. PANNEERSELVAM, P.G. and Research Department of Botany and Microbiology, A.V.V.M. Sri Pushpam College
    (Autonomous) Poondi-613 503,Thanjavur, Tamil Nadu, India.
  • N. THAJUDDIN,Department of Microbiology, Bharathidasan, University, Tiruchirappalli-620 024, Tamil Nadu, India.
  • R. SARAVANA MUTHU,Department of Botany, A.V.C. College, Mayiladuthurai-609 305, Tamil Nadu, India.
  • *Author correspondence: Mobile: 9865412467 E-mail: drmicrsk@yahoomail.com
 

ABSTRACT

 
Cellulolysis rate of the pathogenic fungus, Pestalotia psidii, was determined by estimating the loss in dry weight of filter paper. It varied in relation to changing ferrous iron content of the nutrient solution. There was positive correlation between the growth rate of the fungus and the mean loss in dry weight of the filter paper discs.
 

In-vitro propagation of Pterospermum xylocarpum (Sterculiaceae)

 
  • K.R.S. Sambasiva Rao* ,Department of Biotechnology,JC College of Engineering, Guntur, A.P., India
  • A. Krishna Satya,Centre for Biotechnology, Acharya Nagarjuna University
    Nagarjunanagar – 522 510, Guntur, A.P., India.
  • P. Krishna Kanth,Department of Biotechnology,RV R & JC College of Engineering, Guntur, A.P., India
  • *Author Correspondence: E-mail- krssrao@gmail.com
 

ABSTRACT

 
The present study is focused on in-vitro propagation of a threatened plant of Andhra Pradesh –
Pterospermum xylocarpum (Sterculiacea). Lack of organized cultivation of these plants demand immediate conservative measures. The objectives of the present study are to standardize in-vitro germination techniques, developing a proper in-vitro regeneration protocol using suitable seedling explants, and to obtain organogenesis. The process of micro propagation was carried out by using in-vitro grown seedling explants. Germination studies were carried out to indentify this best medium for developing a healthy seedling. Explant evolution was done upon the shoot regenerative capacity of the inoculated seedling explants. Selected explants were subjected to media evaluation. Morphogenic response of the explants were checked on MS media supplemented with cytokinin – like N6 – Benzylaminopurine (BAP), Kinetin (KN), Thidiazuron (TDZ) and Auxin – like Indole acetic acid (IAA), Naphthalene acetic acid (NAA) and Indole butyric acid (IBA) were supplemented either individually or in combination. The regenerated shoots were separated and placed on the rooting medium augmented with different Auxins like IAA, IBA & NAA (0.1-2 mgL-1). IAA (2 mgL-1) to induce roots from the cut end of the micro shoots. Rooted plantlets were acclimatized to field conditions by placing in pots containing sterilized sand. Cotyledonary nodes of Pterospermum xylocarpum are the best explants for in-vitro propagation. MS medium with 2-3% sucrose was found to be most suitable. Shoot proliferation was maximum in MS medium reinforced with BAP 2mgL-1 + KN 1mgL-1 + 3% sucrose solution. Adventitious rooting was accelerated in micro shoots with IAA at 2mgL-1. Shoot buds are produced from the callus of shoot tips in MS medium reinforced with BAP 4mgL-1 and IAA 1mgL-1.
 

PREVALENCE OF MICROBIAL INHABITANTS FROM COWSHED AND ITS CONTROL

 
  • Bhakyaraj R*, , Arun Sasi, N. Yogananth, A. Chanthuru,
    P.G. and Research Department of Microbiology, J.J. College of Arts and Science,
    Pudukkottai- 622 404, Tamil Nadu, India.
  • J. Vetriselvi,Department of Microbiology, Sengamalathayar College of Arts and Science (Women) Mannarkudi, Tamil Nadu, India.
  • S. Palanivel,Department of Botany, Govt. College of Arts and Science, Karur, Tamil Nadu, India.
  • * Author correspondence: E-mail: rbrajbhakya@gmail.com
 

ABSTRACT

 
Cow dung contains vast quantities of pathogenic bacteria and fungi. Totally four bacterial strains viz; Streptococcus sp, Bacillus sp, Clostridium sp, Staphylococcus sp and five fungal strains viz; Fusarium semitectum, Aspergillus flavus, Aspergillus versicolor, Aspergillus ustus, Aspergillus sydowi were isolated from cow dung with soil and two dominant types of bacteria i.e., Streptococcus sp, Bacillus sp and two fungi i.e., Fusarium semitectum, Aspergillus flavus were chosen for treatment with Na2Co3¬. The experimental group showed a higher inhibition spectra in both bacterial and fungal colonies compared with those in the to control groups.
 

Studies on Production of antibiotic from Tsunami soil isolates of Streptomyces sp at Velanganni, Nagapattinam District

 
  • Chanthuru A*, N.Yogananth, R.Bhakyaraj, Arun Sasi
    P.G. and Research Department of Microbiology, J.J. college of Arts and Science, Pudukkottai- 622 422, Tamil Nadu, India.
  • S.Palanivel,Lecturer, Department of Botany, H.H. Rajah’s college for Men, Pudukkottai, Tamil Nadu, India.
  • *Author for correspondence: E-mail: bioyogaa@gmail.com E-mail: ssspvrm@gmail.com
 

ABSTRACT

 
Tsunami is usually generated by Sea-floor disturbances associated with volcanic eruptions or land slides. Soil samples were collected from tsunami affected areas and their physico-chemical characters analysed to assess the distribution of bacteria and fungi. It was observed that Tsunami soil has toxic chemicals such as Fe, Pb, Hg etc. A total of 5 genera of bacteria viz; Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Bacillus cereus and Bacillus subtilis and one fungi, Candida albicans were isolated. In addition to these, Actinomycetes members were also enumerated. The chloroform extracts of Streptomyces culture filtrates (50 ppm) were screened for antimicrobial activity. Two streptomyces isolates were used. It was observed that Candida albicans and Pseudomonas aeruginosa were highly sensitive (0.0 to 58.0 mm and 25.00 to 60.81 mm respectively), followed by Bacillus cereus (28 to 42 mm) and Staphylococcus aureus (27.0 to 39.62 mm). Inhibitory activity of these isolates appeared to be high for Escherichia coli (10.00 to 30.62 mm) when compared to Bacillus subtilis (11 to 28.62 mm).
 
 

DOES CADMIUM TOXICITY INDUCE OXIDATIVE STRESS IN CHICKPEA (CICER ARIETINUM. L)?

 
  • D Amirtham* and Dr V N Satakopan
    Department of Biochemistry, PSG College of Arts & Science
    Coimbatore, Tamil Nadu, 641014, India
  • *Author for correspondence: E-mail:amirtham_d@yahoo.com
 

ABSTRACT

 
This investigation was carried out to verify whether Cadmium toxicity could cause oxidative stress in Chickpea. The data indicated decreased germination percentage, root length, shoot length and protein level. Significant accumulation of Proline, an indicator of water stress occurred in the presence of Cadmium along with a gradual increase in the activity of antioxidant enzyme SOD. Increased lipid peroxidation (TBARS) was suggestive of disturbed oxidative metabolism. Cadmium caused a transient depletion of Peroxidase and vitamin C in root, shoot and cotyledon. The data also confirmed the inhibitory effect of Cadmium on Glutathione reductase and Glucose 6-phosphate dehydrogenase activities especially in the root system of chickpea. But there is a modified pattern of Gutathione reductase and Glucose 6-phosphate dehydrogenase activity was found in the shoot and cotyledon system suggesting the possibility for the presence of isoenzymes. There was no significant change in the shoot Catalase activity but the root and cotyledon Catalase activities were found to be significantly decreased in presence of Cadmium. Results obtained suggested an induction of oxidative stress in germinating chickpea under Cadmium phytotoxicity.
 

Production of Alpha-amylase enzyme in submerged fermentation by using Bacillus sp

 
  • Arun Sasi*, Bhakyaraj. R, Yogananth. N, Chanthuru. A,
    P.G. & Research Department of Microbiology
    J.J. College of Arts and Science, Pudukkottai – 622 422.
  • Ravikumar. M ,Department of Plant Biology and Plant Biotechnology Govt. Arts College for Men, Nandanam, Chennai.
  • *Author correspondence: E-mail- vavamicro@gmail.com
 

ABSTRACT

 
Amylases (endo-1,4?-D glucan glycohydrolases EC 3.2.1.1) are enzymes that hydrolyze starch. They can be derived from several sources such as plants, animals and microorganisms. They are used in food, baking, brewing, detergent, textile, paper and distilling industries. ?-amylase enzyme can be produced in submerged fermentation by using Bacillus substilis and Bacillus lencheniformis. The Minimal medium was supplemented with certain carbon sources like fructose, glucose, lactose, mannitol, mannose and sucrose, certain other nitrogen compounds like peptone, urea, (NH4)2 SO4, NaNO3 and yeast extracts and certain heavy metal compounds like Ca 2+, Mg 2+, Zn 2+, Mn 2+, Fe 2+ and Co 2+. In this study it was found that B. substilis produced more amounts of amylase (278 ?l –1) than B. lencheniformis in both normal and supplemented media in 37°C at pH 7.
 

Deterioration of fabric paints by two species of Aspergillus, Tham and Raper.

 
  • Malathy NS* and KR Deepa
    Department of Botany, PSGR Krishnammal College for Women, Coimbatore-641004.
  • *Author correspondence: Mobile-9944562193, E-mail: malathy.ns@gmail.com
 

ABSTRACT

 
Aspergillus flavus and Aspergillus .fumigatus were isolated from spoilt black and yellow fabric paints respectively. The capacity of these two fungi to utilize fabric paints for their growth was studied by inoculating them on fresh paints and on Czapex-Dox medium with and without sucrose but containing 50, 250 and 500 ppm concentrations of black and yellow fabric paints. Both fungi could grow to some extent directly on fresh black and yellow fabric paints. In Czapek-Dox medium with sucrose and 50ppm concentration of either black or yellow fabric paints they grew well and sporulated profusely. In Czapek-Dox medium without sucrose but with different concentrations of paints as carbon source the fungi showed slightly more growth than the control indicating that they utilize the chemicals present in paints. Zinc oxide, a common fungistatic substance commonly used in paints was found to be ineffective in controlling A.flavus and A.fumigatus at the concentration routinely used in paints.
 
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